recombinant proteins recombinant avb8 r  (R&D Systems)

Journal: Cell reports

Article Title: Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy

doi: 10.1016/j.celrep.2021.109309

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant avb8 , R and D Systems , 4135-AV-050.

Techniques: Recombinant, Software

Journal: Journal of the American Society of Nephrology

Article Title: Pharmacologic Blockade of αvβ1 Integrin Ameliorates Renal Failure and Fibrosis In Vivo

doi: 10.1681/asn.2015050585

Figure Lengend Snippet: Figure 1. Renal fibroblasts express multiple av integrins and activate TGF-b. (A) Tdtomato- positive kidney cells isolated from PDGFRb-Cre, Ai14tdtomatoFlox/Flox mice and differen- tiated in culture were subjected to flow cytometry analysis. Cells were incubated with either secondary antibody alone (control, in black) or first with mouse mAbs (in gray) for avb3 (Axum-4), avb5 (Alula), avb6 (3G9), or avb8 (Adwa11), all generated by immunizing knockout mice with integrin subunits. (B) Co-IP and WB of cell lysates of isolated Tdtomato- positive mouse kidney cells show that these cells express avb1, avb3, and avb5. Cell ly- sates were immunoprecipitated with anti-av antibodies (RMV-7) or isotype control rat IgG1k. Each immunoprecipitate was divided in half and each half was subjected to WB with antibodies against av and a respective b integrin subunit. TL, total lysates. (C) Isolated Tdtomato-positive cells were used in coculture TGF-b activation assay in the presence or absence of blocking antibodies against av (rat monoclonal IgG1k), b1 (hamster mono- clonal IgG1k), b3 (hamster monoclonal IgG1k), or avb5 (mouse monoclonal IgG). *P,0.05, specific antibody versus corresponding isotype control-treated samples). hIgG, hamster IgG1k; mIgG, mouse IgG; rIgG, rat IgG1k. (D) TGF-b activation assays with isolated Tdtomato-positive cell in the presence of C8 (line with black markers) or inactive control compound C16 (line with gray markers) at indicated concentrations. No Rx, no treatment. For both (C and D), relative TGF-b activation is expressed as percentage luminescence of each treatment sample compared with untreated samples after subtraction of TGF-b–independent luminescence.Datashownarethemean6SEMfromthreeexperiments.

Article Snippet: Mouse monoclonal avb8 antibody Adwa11 was generated by injecting Itgb82/2 mice with recombinant human avb8 protein (Cat#4135-AV-050; R&D Systems).

Techniques: Isolation, Cytometry, Incubation, Control, Generated, Knock-Out, Co-Immunoprecipitation Assay, Immunoprecipitation, Activation Assay, Blocking Assay